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YAC-1
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JY Djeu
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Biosafety Level:
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2
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frozen
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See Propagation
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suspension
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Mus musculus (mouse)
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lymphoblast
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Disease: lymphoma
Strain: A/Sn
Cell Type: Moloney murine leukemia virus (Mo-MuLV) induced
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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assay of NK cell activity ( [22345] [49299] The cells are sensitive to the action of natural killer (NK) cells and are useful in assays of NK cell activity.)
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
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Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 10(5) cells/ml and maintain between 2 X 10(5) and 2 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
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Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
Cell culture tested DMSO:ATCC 4-X
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22345: Kiessling R, et al. "Natural" killer cells in the mouse. I. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Specificity and distribution according to genotype. Eur. J. Immunol. 5: 112-117, 1975. PubMed: 1234049
26348: Cikes M, et al. Progressive loss of H-2 antigens with concomitant increase of cell- surface antigen(s) determined by Moloney leukemia virus in cultured murine lymphomas. J. Natl. Cancer Inst. 50: 347-362, 1973. PubMed: 4573851
32552: Okazaki IJ, et al. Cloning and characterization of a novel membrane-associated lymphocyte NAD:arginine ADP-ribosyltransferase. J. Biol. Chem. 271: 22052-22057, 1996. PubMed: 8703012
49299: Kiessling R, et al. "Natural" killer cells in the mouse. II. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Characteristics of the killer cell. Eur. J. Immunol. 5: 117-121, 1975. PubMed: 1086218
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