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產品名稱：CRL-2755 EMT6 小鼠乳腺癌細胞
產品型號：CRL-2755
產品廠商：美國標準生物品收藏中心（ATCC）
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CRL-2755 EMT6 小鼠乳腺癌細胞，ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞；細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和培養條件！

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CRL-2755 EMT6 小鼠乳腺癌細胞

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CRL-2755™

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Designations:	EMT6
Depositors:	S Rockwell
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Mus musculus (mouse)
Morphology:	epithelial
Source:	Organ: breast Strain: BALB/cCrgl Disease: mammary carcinoma Cell Type: epithelial
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation:	Isolation date: 1971
Tumorigenic:	Yes
Gender:	female
Comments:	EMT6 was established from a transplantable murine mammary carcinoma that arose in a BALB/cCRGL mouse after implantation of a hyperplastic mammary alveolar nodule. The resulting tumor line (named KHJJ) was propagated in BALB/cKa mice and adapted to tissue culture after the 25th animal passage, and the cell line was named EMT. EMT6 is a clonal isolate of EMT isolated in 1971 at Stanford University. The EMT6 cell line can be grown either in animals as a tumor or in tissue culture. Cells derived from tumors have a reported in vitro plating efficiency of 30%. Cell grown in tissue culture reportedly have a plating efficiency of 70%.
Propagation:	ATCC complete growth medium: Waymouth's MB 752/1 Medium with 2mM L-glutamine, 85%; fetal bovine serum, 15% Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
Subculturing:	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: Every 2 to 3 days
Preservation:	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Related Products:	recommended serum:ATCC 30-2020
References:	61342: Rockwell SC, et al. Characteristics of a serially transplanted mouse mammary tumor and its tissue-culture-adapted derivative. J. Natl. Cancer Inst. 49: 735-749, 1972. PubMed: 4647494 61343: Palom Y, et al. Structure of adduct X, the last unknown of the six major DNA adducts of mitomycin C formed in EMT6 mouse mammary tumor cells. Chem. Res. Toxicol. 13: 479-488, 2000. PubMed: 10858321 61344: Collingridge DR, Rockwell S. Pentoxifylline improves the oxygenation and radiation response of BA1112 rat rhabdomyosarcomas and EMT6 mouse mammary carcinomas. Int. J. Cancer 90: 256-264, 2000. PubMed: 11091349 61345: Rockwell S, Kelley M. RSR13, a synthetic allosteric modifier of hemoglobin, as an adjunct to radiotherapy: preliminary studies with EMT6 cells and tumors and normal tissues in mice. Radiat. Oncol. Investig. 6: 199-208, 1998. PubMed: 9822166 61346: Rockwell S. In vivo-in vitro tumor systems: new models for studying the response of tumors to therapy. Lab. Anim. Sci. 27: 831-851, 1977. PubMed: 338981 70368: Rockwell STumor-Cell SurvivalIn: Rockwell STumor models in cancer researchTotowa, New JerseyHumana Press Inc.617-631.

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產品名稱：CRL-2755 EMT6 小鼠乳腺癌細胞