Organ: spleen Strain: BALB/c Cell Type: hybridoma: B lymphocyte;
Permits/Forms:
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:
fusion partner for production of somatic cell hybrids
Antigen Expression:
H-2d
Comments:
The line was formed by fusing BALB/c spleen cells (from mouse immunized with sheep RBCs) with the P3X63Ag8 myeloma. [1077]
The cells do not secrete immunoglobulin, are resistant to 8-azaguanine at 20 mcg/ml and are HAT sensitive. [1077]
Sp2/0-Ag14 cells can be used as fusion partners for B cells in the production of hybridomas. [906]
Tested and found negative for ectromelia virus (mousepox).
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C
Subculturing:
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Maintain cell density between 5 X 10 (4) and 5 X 10 (5) viable cells/ml. Some cells can attach and be transferred by shaking them loose. Medium Renewal: Add fresh medium every 2 to 4 days (depending on cell density)
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
derivative:ATCC CRL-1581.1
References:
906: Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J. Immunol. 126: 317-321, 1981. PubMed: 6935293 1077: Shulman M, et al. A better cell line for making hybridomas secreting specific antibodies. Nature 276: 269-270, 1978. PubMed: 714156 32373: Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260 33167: Chen H, et al. Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J. Biol. Chem. 271: 15743-15752, 1996. PubMed: 8663022
)
